Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans.
نویسندگان
چکیده
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripheral blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1 micron diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripheral blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
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ورودعنوان ژورنال:
- Revista da Sociedade Brasileira de Medicina Tropical
دوره 30 6 شماره
صفحات -
تاریخ انتشار 1997